The Marathon route is of low difficulty. Its main characteristics are the small altitude fluctuations and the long straight street that make it ideal for great records. Some other characteristic features are its constant east direction, the long straight street and the limited inhabited areas which it crosses until almost the 30th kilometer.
The race starts from Pella, in front of the statue of Alexander the Great on horseback, hardly a km from the homonym archaeological site, and some 43 meters above sea-level. After the first km of downhill road, the athletes get onto the Thessaloniki-Edessa highway, following it for another 8 km, until they reach Chalkidona.